2025小妹全国空降,全国高端商务伴游,如何寻找接私活的女生微信,附近学生24小时随叫随到手机号码

MMP13 - catalytic domain, inactive mutant
货号：G04MP13Ci
品牌：Giotto Biotech
规格：10 μg
目录价：询价
商品详情 相关下载

Description

Description
MW = 18.6 kDa. Recombinant matrix metalloproteinase-13 (MMP-13, collagenase-3, col3) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-13 (residues 104-268, UniProtKB accession P45452). The enzyme has been inactivated by mutagenesis (E223A). This product is derived from MMP-13 Catalytic domain and contain also the mutation F175D to increase its stability. No residual enzyme activity has been detected using the method described in the specific activity section of this data sheet.
 
Sequence 
       110        120        130        140        150
   YNVFPRT LKWSKMNLTY RIVNYTPDMT HSEVEKAFKK AFKVWSDVTP
       160        170        180        190        200 
LNFTRLHDGI ADIMISFGIK EHGDDYPFDG PSGLLAHAFP PGPNYGGDAH
       210        220        230        240        250 
FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYTYTGKS
       260        
HFMLPDDDVQ GIQSLYGP
 
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight of about 18.4 kDa.
 
Supplied as
0.2 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 29910 M-1cm-1 calculated).
 
Specific activity
Not detected. Activity described as U=100 pmol/min at 25°C using a colorimetric ***** with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5(Biomol) as substrate.

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

尼玛县ps5772| 盘锦市ast663| 仪陇县y6t360| 三穗县ihn708| 石景山区4hx981| 吉林省pq4771| 大庆市juv683| 湟源县o4j875| 文安县gpq216| 东海县4tw543| 日照市gt5473| 泸州市uld107| 湘潭县wao382| 晋城i5g138| 吉林市pqy906| 柳河县5yw997| 儋州市ka3247