100 110 120 130 140 M-YSLFPN SPKWTSKVVT YRIVSYTRDL PHITVDRLVS KALNMWGKEI 150 160 170 180 190 PLHFRKVVWG TADIMIGFAR GAHGDSYPFD GPGNTLAHAF APGTGLGGDA 200 210 220 230 240 HFDEDERWTD GSSLGINFLY AATHELGHSL GMGHSSDPNA VMYPTYGNGD 250 260 PQNFKLSQDD IKGIQKLYGK RSNSRKK
Supplied As 0.1 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 33920 M-1cm-1 calculated).
Specific activity > 8U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric ***** with thiopeptide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
Storage -80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C. Avoid repeated freeze/thaw cycles.
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